Key Responsibilities

Organoid and Spheroid Platform Development

- Develop, optimize, and characterize tissue-specific 3D spheroid and organoid models derived from primary cells, stem cells, and relevant human cell sources.

- Establish reproducible protocols for organoid generation, expansion, maintenance, differentiation, and cryopreservation.

- Design and implement disease-relevant in vitro models for evaluating regenerative and therapeutic interventions.

- Develop novel 3D culture methodologies and improve platform robustness, scalability, and reproducibility.

- Perform phenotypic, molecular, and functional characterization of organoids and spheroids.

Assay Development and Validation

- Develop and validate medium- to high-throughput cell-based assays for biomarker quantification and therapeutic efficacy assessment.

- Design and execute functional assays related to tissue regeneration, inflammation, fibrosis, angiogenesis, wound healing, and cellular function.

- Optimize assay workflows and establish qualification criteria for reproducibility and robustness.

- Support assay transfer, standardization, and technology implementation across teams.

Cell and Molecular Biology

- Perform mammalian cell culture, primary cell culture, stem cell culture, and differentiation studies.

- Conduct molecular and biochemical analyses including:

- ELISA and multiplex cytokine assays

- RNA isolation and purification

- RT-qPCR and gene expression analysis

- Protein quantification assays

- Western blotting

- Immunocytochemistry and immunofluorescence

- Flow cytometry (preferred)

- Support sample preparation and analysis for microscopy-based studies including phase contrast, fluorescence, confocal, and high-content imaging.

Histology and Tissue Characterization

- Perform tissue processing, fixation, paraffin embedding, cryo-embedding, microtomy, and cryosectioning of organoids, spheroids, and engineered tissue constructs.





- Conduct histological characterization using H&E; and relevant special stains.

- Perform immunohistochemistry (IHC) and immunofluorescence (IF) for tissue-specific marker analysis.

- Analyze tissue architecture, differentiation status, cellular organization, and biomarker expression in 3D tissue models.

- Support quantitative image analysis and histology assessments.

Data Analysis and Scientific Reporting

- Design experiments and perform data acquisition, processing, statistical analysis, and interpretation.

- Prepare technical reports, study summaries, scientific presentations, and publication-quality figures.

- Maintain accurate experimental records and documentation.

- Contribute to scientific publications, patents, grant proposals, and regulatory documentation.

Project Execution and Collaboration

- Independently design and execute R&D; projects aligned with organizational goals.

- Mentor and train Research Associates and junior scientists.

- Coordinate activities across multidisciplinary teams including biology, process development, analytics, translational research, and product development.

- Participate in scientific discussions, project reviews, and strategic planning initiatives.

Quality Systems and Documentation

- Develop, optimize, and maintain SOPs, protocols, and experimental workflows.

- Ensure compliance with laboratory safety practices and internal quality standards.

- Support continuous improvement initiatives and implementation of best laboratory practices.





Educational Qualifications

- PhD in Cell Biology, Molecular Biology, Biotechnology, Biochemistry, Regenerative Medicine, Tissue Engineering, Biomedical Sciences, or a related field.

Experience

- Minimum 1–2 years of relevant postdoctoral and/or industrial research experience.

- Prior experience in organoid, spheroid, tissue engineering, or disease modeling platforms is preferred.

- Experience working in an industry R&D; environment is preferred.

Mandatory Technical Expertise

- Extensive hands-on experience in:

- 3D organoid and/or spheroid culture systems

- Mammalian, primary, and stem cell culture

- Stem cell differentiation and tissue model development

- Disease modeling and cell-based functional assays

- Histology workflows including tissue processing, embedding, microtomy, cryosectioning, H&E; staining, IHC, and IF

- RT-qPCR, ELISA, immunostaining, and molecular biology techniques

- Confocal and fluorescence microscopy

- Experimental design, data analysis, and statistical interpretation

Preferred Skills

- Experience in regenerative medicine, tissue engineering, and translational research.

- Experience with high-content imaging systems and image analysis software.

- Familiarity with biomarker discovery and mechanism-of-action studies.

- Experience supporting preclinical research programs.

- Demonstrated scientific productivity through publications, patents, technology development, or grant participation.

Desired Attributes

- Strong scientific curiosity and innovation-driven mindset.

- Excellent experimental troubleshooting and problem-solving abilities.

- Ability to independently manage research projects and timelines.

- Strong communication, presentation, documentation, and mentoring skills.

- Ability to work effectively within multidisciplinary teams in a fast-paced research setting.

📌 Associate Research Scientist - Organoid (Bengaluru)
🏢 Pandorum Technologies
📍 Bengaluru