Responsibilities
Enzyme Screening & Strain Improvement: Isolate, screen, and characterize microbial strains (fungal/bacterial) for high-yield enzyme production; conduct classical mutagenesis or recombinant strain evaluation.
Fermentation & Process Optimization: Execute laboratory and bench-scale fermentation (Shake Flask & Bioreactor / Fermenter up to 5L-10L) to optimize growth parameters, media formulations, and yield metrics for target enzymes.
Downstream Processing (DSP): Perform basic downstream purification techniques including cell disruption, filtration, precipitation, ultrafiltration, and chromatography to isolate active enzyme proteins.
Enzyme Characterization & Assays: Develop, validate, and perform enzymatic assays to determine enzyme activity, kinetic parameters ($Km$, $V{max}$), temperature/pH stability profiles, and substrate specificity.
Application Testing: Evaluate enzyme performance under simulated commercial conditions for target industries (e.g., starch hydrolysis, baking, brewing,



textile desizing, or protein hydrolysis).
Scale-Up & Technology Transfer: Prepare Standard Operating Procedures (SOPs), Technology Transfer Dossiers (TTD), and coordinate with Pilot Plant / Production teams during process scale-up.
Documentation & Safety: Maintain accurate laboratory notebooks, record experimental data in compliance with Valuable Laboratory Practices (GLP), and ensure secure handling of biological agents and hazardous chemicals.

Requirements
Experience 1 - 2 Years
Industry Engineering / Engineering Design / R&D; / Quality Qualification M.Pharma

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📌 R&d Executive / Officer Enzymes Nashik
🏢 Impact Hr N Km Solutions
📍 Nashik