Key Responsibilities

Organoid and Spheroid Platform Development

Develop, optimize, and characterize tissue-specific 3D spheroid and organoid models derived from primary cells, stem cells, and relevant human cell sources.
Establish reproducible protocols for organoid generation, expansion, maintenance, differentiation, and cryopreservation.
Design and implement disease-relevant in vitro models for evaluating regenerative and therapeutic interventions.
Develop novel 3D culture methodologies and improve platform robustness, scalability, and reproducibility.
Perform phenotypic, molecular, and functional characterization of organoids and spheroids.
Assay Development and Validation

Develop and validate medium- to high-throughput cell-based assays for biomarker quantification and therapeutic efficacy assessment.
Design and execute functional assays related to tissue regeneration, inflammation, fibrosis, angiogenesis, wound healing, and cellular function.
Optimize assay workflows and establish qualification criteria for reproducibility and robustness.
Support assay transfer,



standardization, and technology implementation across teams.
Cell and Molecular Biology

Perform mammalian cell culture, primary cell culture, stem cell culture, and differentiation studies.
Conduct molecular and biochemical analyses including:
ELISA and multiplex cytokine assays
RNA isolation and purification
RT-qPCR and gene expression analysis
Protein quantification assays
Western blotting
Immunocytochemistry and immunofluorescence
Flow cytometry (preferred)
Support sample preparation and analysis for microscopy-based studies including phase contrast, fluorescence, confocal, and high-content imaging.
Histology and Tissue Characterization

Perform tissue processing, fixation, paraffin embedding, cryo-embedding, microtomy, and cryosectioning of organoids, spheroids, and engineered tissue constructs.
Conduct histological characterization using H&E; and relevant special stains.
Perform immunohistochemi

📌 Associate Research Scientist (Bengaluru)
🏢 Pandorum Technologies
📍 Bengaluru