We are looking for a talented Molecular Biologist to join our team and contribute to groundbreaking mRNA vaccine development. The ideal candidate will have experience in molecular and cell biology techniques, including DNA and RNA extraction, PCR, cloning methods, cell line maintenance, transfection, protein expression, and protein purification. You will play a critical role in advancing our mRNA and protein-based therapeutics pipeline, working across multiple areas of our research and development efforts.
Key Responsibilities:
- Perform DNA and RNA extraction and plasmid extraction at various scales.
- Execute vector transformation in bacterial strains such as DH5-Alpha or similar.
- Apply a wide range of PCR techniques including inverse, touch down/up, gradient, long amplicons, TaqMan, and real-time PCR for various applications.
- Carry out cloning via methods like cut-and-paste, Gibson assembly, Gateway, and In-Fusion.
- Handle, maintain, and stock various cell lines , particularly HEK 293T, HeLa, MCF-7, and suspension cell lines such as Expi and FreeStyle series.




- Perform transfection using both chemical and electroporation methods at varying scales.
- Achieve protein expression in bacteria , yeast , insect , and mammalian cells , and support protein separation and purification (optional).
- Conduct protein estimation assays (e.g., Bradford, BCA, or ELISA).
- Use Western blotting for estimating relative protein expression levels.
- Utilize FPLC systems such as Cytiva's Akta series or Bio-Rad's NGC line (desirable but not mandatory).
- Collaborate with cross-functional teams to advance various R&D; projects in mRNA vaccine development, cell line generation, and protein production.

Qualifications:
- PhD in Molecular Biology, Cell Biology, or a related field; OR a Master's degree in a relevant field with at least 3+ years of additional experience in a relevant laboratory setting.
- Hands-on experience with DNA and RNA extraction , as well as plasmid extraction

📌 Molecular & Cell Biologist (Secunderabad)
🏢 University Of Cambridge
📍 Secunderabad