Candidate Responsible For
Cloning of genes in E.coli, insect and mammalian expression vectors and sequence verification.
Perform and optimize protein expression studies in E.coli and small scale purification by affinity chromatography.
Secure cell line development in mammalian cells, starting from transfection to cell banking and expression parameters optimization.
Protein expression in Insect cell lines
Document the experiments in timely manner and maintain the records.
Timely transfer of the process/technology to respective department.
Work alongside the seniors in contributing to overall business objective.