Research Executive- Animal Cell Culture Responsibilities

• Perform routine maintenance of animal cell lines, including cell thawing, revival, subculturing (passaging), and cryopreservation.

• Prepare complete culture media, buffers, reagents, and supplements under aseptic conditions.

• Maintain adherent and suspension cell cultures in a sterile environment using Biosafety Cabinets (BSC) and CO₂ incubators.

• Perform cell counting and viability analysis using Trypan Blue exclusion assay and hemocytometer.

• Monitor cell morphology, growth, and confluency using an inverted microscope and maintain healthy cell cultures.

• Perform contamination detection and prevention, including monitoring for bacterial, fungal, and mycoplasma contamination.

• Carry out cell-based assays such as MTT assay, Cell Viability Assay, Cell Proliferation Assay, Cytotoxicity Assay, Live/Dead Cell Assay, and Apoptosis Assays.

• Perform sample preparation for flow cytometry, fluorescence microscopy, and other downstream molecular analyses.

• Handle, label, store, and transport biological samples, cell lines, and reagents according to laboratory SOPs and biosafety guidelines.

• Participate in research planning, literature review, experimental design, and optimization of cell culture protocols.

• Maintain accurate laboratory notebooks, cell culture logs, inventory records, equipment maintenance records, and project documentation.

• Follow Valuable Cell Culture Practices (GCCP), Good Laboratory Practices (GLP), biosafety regulations, and quality control procedures.

• Learn and implement new cell culture techniques, SOPs,



and experimental protocols to support ongoing research projects.

• Ensure ethical handling of biological materials and maintain confidentiality of research data and project information.

• Collaborate with cross-functional teams to support research, product development, validation studies, and client projects.

Research Executive- Genomics Responsibilities

• Performing DNA/RNA isolation, purification and characterization of samples.

• Performing RT-PCR, Real time PCR and PCR amplifications for analysis of gene expression with gel e.

• Performing nucleic acid characterization with Gel electrophoresis (both qualitative and quantitative).

• Sample preparation for Sequencing and NGS analysis

• Handling, storage, and packaging of samples (raw and processed) as per guidelines.

• Participation in planning and literature survey of the projects.

• Create specific strategies for easier and effective execution of projects.

• Keep records of all information related to project for documentation, clarification and presentation to management

• Learning new project SOPs and adding value Enhance department reputation by accepting ownership for accomplishing new and different requests; exploring opportunities to add value to job accomplishments.

• Adhere to ethical guidelines and standards for conducting research and handling data.

• Protect sensitive information and maintain confidentiality.

• Ensure the accuracy, reliability, and validity of research data and findings.

• Implement quality control measures to maintain research standards.

📌 Research Executive (Lucknow)
🏢 Aakaar Biotechnologies Private
📍 Lucknow