Research Assistant Stem Cells and Proteomics

Research Overview Pathologically, PD is characterized by the degeneration of dopaminergic neurons in the substantia nigra pars compacta and the accumulation of alpha-synuclein in Lewy bodies. This neuronal loss may arise from intrinsic neuronal dysfunction and/or altered glial cell function. Understanding these early cellular and molecular events is essential for identifying key drivers of neurodegeneration. A major challenge in studying these mechanisms is the limited availability of robust human model systems that accurately capture early disease processes and cell-type-specific contributions. Patient-derived induced pluripotent stem cell (iPSC) technology now enables modelling of neuronal and glial dysfunction using 2D monocultures, as well as neuron glia interactions through advanced 3D organoid systems. In addition,



CRISPR/Cas-based genome editing approaches are being used to investigate the functional impact of PD-associated mutations by introducing disease-causing variants into control iPSC lines and generating isogenic controls through gene correction in patient-derived iPSC lines. The project will involve team-oriented proteomics studies to uncover molecular mechanisms underlying neuron-glia crosstalk in PD. We are seeking a motivated and skilled Research Assistant with experience in Reprogramming technology, iPSC culture and differentiation techniques. The candidate will also be responsible for coordinating with proteomics collaborators and contributing to the analysis and interpretation of proteomics datasets to identify disease relevant molecular pathways.

Mandatory Requirements
- Experience in reprogramming PBMCs to generate iPSCs
- Hands-on experience in culturing, passaging, freezing, and thawing iPSC/ESC lines
- Experience in media preparation for iPSC-based differentiation protocols specially neuronal and glial differentiation
- Experience in transfection techniques such as Electroporation, nucleofectio

📌 Research Assistant Stem Cells and Proteomics (Bengaluru)
🏢 SKAN Research Trust
📍 Bengaluru